Clinical Guide: Toluidine Blue Dye

There’s been very little written about TB dye in the peer-reviewed literature over the past 30 years, so I have included all of it (that I could find) here. Some discuss the utility of TB dye in greater depth than others. As with any research, it is imperative that you read these articles (particularly the older ones) with a critical eye toward the methodology used and the relevance of their findings in light of advances in the science.

FHO Articles

Interesting Conversations About Toluidine Blue Dye Use

Interesting Conversations About Toluidine Blue Dye Use

With the interesting conversation about toluidine blue dye use going on over at the IAFN community site, a quick reminder, we have a clinical guide on that topic here at FHO. There seems to be some disagreements about where it can be…

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Articles (Full-text and abstracts)

Comparison of methods for identifying ano-genital injury after consensual intercourse

Zink T, Fargo JD, Baker RB, Buschur C, Fisher BS, Sommers MS. Comparison of methods for identifying ano-genital injury after consensual intercourse. (FULL-TEXT). J Emerg Med. 2010 Jul;39(1):113-8. doi: 10.1016/j.jemermed.2008.08.024.
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Effects Of Toluidine Blue And Destaining Reagents Used In Sexual Assault Examinations On The Ability To Obtain DNA Profiles From Postcoital Vaginal Swabs

Hochmeister MN, Whelan M, Borer UV, Gehrig C, Binda S, Berzlanovich A, Rauch E, Dirnhofer R. Effects Of Toluidine Blue And Destaining Reagents Used In Sexual Assault Examinations On The Ability To Obtain DNA Profiles From Postcoital Vaginal Swabs. J Forensic Sci. 1997 Mar;42(2):316-9.
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Factors That Influence The Variability In Findings Of Anogenital Injury In Adolescent/Adult Sexual Assault Victims: A Review Of The Forensic Literature

Laitinen FA, Grundmann O, Ernst EJ. Factors That Influence The Variability In Findings Of Anogenital Injury In Adolescent/Adult Sexual Assault Victims: A Review Of The Forensic Literature. Am J Forensic Med Pathol. 2013 Sep;34(3):286-94. doi: 10.1097/PAF.0b013e31829a0f46.
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Reliability Of Female Genital Injury Detection After Sexual Assault

Sachs CJ, Benson A, Schriger DL, Wheeler M. Reliability Of Female Genital Injury Detection After Sexual Assault. J Forensic Nurs. 2011 Dec;7(4):190-4. doi: 10.1111/j.1939-3938.2011.01117.x.
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Toluidine Blue In The Detection At Autopsy Of Perineal And Anal Lacerations In Victims Of Sexual Abuse

Bays J, Lewman LV. Toluidine Blue In The Detection At Autopsy Of Perineal And Anal Lacerations In Victims Of Sexual Abuse. Arch Pathol Lab Med. 1992 Jun;116(6):620-1.
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Toluidine Blue In The Detection Of Perineal Lacerations In Pediatric And Adolescent Sexual Abuse Victims

McCauley J, Gorman RL, Guzinski G. Toluidine Blue In The Detection Of Perineal Lacerations In Pediatric And Adolescent Sexual Abuse Victims. Pediatrics. 1986 Dec;78(6):1039-43.
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Significance Of Toluidine Blue Positive Findings After Speculum Examination For Sexual Assault

Jones JS, Dunnuck C, Rossman L, Wynn BN, Nelson-Horan C. Significance Of Toluidine Blue Positive Findings After Speculum Examination For Sexual Assault. Am J Emerg Med. 2004 May;22(3):201-3.
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Signs of genital trauma in adolescent rape victims examined acutely

Adams JA, Girardin B, Faugno D. Signs of genital trauma in adolescent rape victims examined acutely. J Pediatr Adolesc Gynecol. 2000 May;13(2):88.
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Use Of Toluidine Blue For Documentation Of Traumatic Intercourse

Lauber AA, Souma ML. Use Of Toluidine Blue For Documentation Of Traumatic Intercourse. Obstet Gynecol. 1982 Nov;60(5):644-8.
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Validation Set Correlates Of Anogenital Injury After Sexual Assault

Drocton P, Sachs C, Chu L, Wheeler M. Validation Set Correlates Of Anogenital Injury After Sexual Assault. Acad Emerg Med. 2008 Mar;15(3):231-8. doi: 10.1111/j.1553-2712.2008.00050.x.
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Books

Atlas of Sexual Violence

Atlas of Sexual Violence (Henry, pp. 100-101)
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Forensic Emergency Medicine (2nd Ed.)

Forensic Emergency Medicine (2nd Ed.). (Olshaker, et al. pp. 99-101)
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Obstetric & Gynecologic Emergencies

Obstetric & Gynecologic Emergencies. (Pearlman, et al., p. 478)
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Forensic Nursing

Forensic Nursing. (Pyrek, p. 164)
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Clinical Forensic Medicine

Clinical Forensic Medicine. (Stark, pp. 92-93).
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Manual of Forensic & Emergency Medicine

Manual of Forensic & Emergency Medicine. (Riviello, pp. 115-116)
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Color Atlas of Sexual Assault

Color Atlas of Sexual Assault (Girardin, et al., pp. 102, 120-121)
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Sexual Assault Victimization Across the Lifespan

Sexual Assault Victimization Across the Lifespan (Girardin, et al. starting on p. 661)
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Sexual Assault

Sexual Assault (Crowley, pp. 85-86)
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